Sweat Testing

Specimen Collection

The Cystic Fibrosis Foundation (CFF) recommends that a sweat test be done at a CFF-accredited care center by a trained technician. The sweat test is typically performed on the patient’s arm or leg. The test involves iontophoresis of pilocarpine to stimulate sweat production by sweat glands. Collection modalities include gauze, filter paper, or macroduct coil.[2] Since the sweat chloride can present as transiently elevated in the first 24 hours of life, the CFF clinical care guidelines recommend that sweat testing should be done after 48 hours of birth or later. Two samples are collected due to the underlying variability of the test and the potential for an insufficient sample. Pilocarpine iontophoresis is done for 5 minutes, followed by sweat collection for not more than 30 min. For the gauze or filter paper method, the stimulated area must be 2 x 2 inches. The minimum quantity of sweat collected for the gauze method is 75 mg, and a minimum sample volume of 15 microliters is necessary for the macroduct method.[2]

Procedures

The clinician conducts iontophoresis of pilocarpine on the patient's skin. In the original Gibson-Cooke method, two electrodes, one covered in gauze soaked with pilocarpine and the other with deionized water, are placed on the patient's arm or leg. A small, painless, electrical current is then applied for 5 minutes; referred to as iontophoresis. Afterward, a collecting source, such as gauze, filter paper, or a Macroduct coil is used to collect the sweat. The allotted time for sweat collection should not exceed 30 minutes. Using the Gibson-Cooke method for QPIT, a minimum of 75mg of sweat is required adequate testing. However, most centers in the US utilize collection via macroduct coil, which requires only 15 microliters of sweat for analysis. Sweat should not be collected from multiple sites for the same test as the rate of sweating determines the chloride content. The collected source then goes to the lab for quantification of chloride.[2][3]

Indications

Indications for the sweat test include individuals suspected of having cystic fibrosis, either through a positive newborn screening test or if clinical features suggestive of CF are present. The sweat test is considered to be the gold standard as a confirmatory test. CF genotyping is recommended to help decide CF mutation-specific therapy, in case the sweat test results are borderline or sweat test is not technically possible (e.g., severe eczema). The CFF recommends CF genotyping in all patients. In the United States, the newborn screen tests for an elevated level of IRT (immunoreactive trypsinogen) and if positive may reflex to a DNA test for known mutations of the CFTR gene, depending on the each state’s protocol. A child demonstrating a positive newborn screen will subsequently require confirmatory sweat testing at a CF center.[3]

In children clinically suspected to have cystic fibrosis, a referral to a CF center for sweat testing is indicated. Patients with cystic fibrosis may present with a myriad of different presentations. Neonates may present with meconium ileus. Smaller children may present with pulmonary complications such as recurrent pneumonia, upper respiratory infections, wheezing, and coughing. Gastrointestinal manifestations include failure to thrive with malabsorptive stools, recurrent abdominal pain. Given the clinical suspicion, children obtain a referral for testing.[4] Table 1 describes some of the common indications for sweat testing. Sweat testing may also serve as a surrogate biomarker for evaluating CFTR function. The result of a sweat test informs clinicians regarding the effects of CFTR-modulating drugs such as ivacaftor.[4]

Potential Diagnosis

An elevated level of chloride (over 60 mmol/L) in the sweat is diagnostic for cystic fibrosis, sweat chloride levels in less than 29mmol/L is normal. A level between 30 to 60 mmol/L is considered borderline, and repeat sweat test or further testing is required. This range may reflect a heterozygous carrier which cannot be accurately detected with sweat testing. The result of a positive newborn screen needs to be interpreted together with the sweat to test to reliably diagnose CF. Specific known mutations of CF do not cause elevated sweat chloride, and therefore sweat testing does not provide clarity.[1]

Patients with a positive newborn screen and a negative sweat test sometimes receive diagnoses other than CF, such as CF-related metabolic syndrome (CRMS) and CF screen-positive, inconclusive diagnosis (CFSPID). A normal sweat chloride test does not exclude the diagnosis, in which case the clinician should pursue genotyping or other alternative diagnostic studies (such as nasal membrane potential difference, intestinal current measurement, semen analysis, or assessment of pancreatic function), especially if there is a high index of clinical suspicion for cystic fibrosis.[1]

Normal and Critical Findings

Sweat chloride levels:

•  Less than 30 mmol/L: CF is unlikely
• 30 to 59 mmol/L: CF is possible, further testing may be required
• 60 or greater mmol/L: diagnostic of CF

Sweat chloride in patients without cystic fibrosis should be less than 30 mmol/L. Values greater than 60 mmol/L are diagnostic of cystic fibrosis. Intermediate values between 30 to 60 mmol/L are considered to be an elevated but not diagnostic level. Cystic fibrosis is possible in these patients, but repeat or alternative testing is necessary.[1]

For patients with intermediate sweat chloride levels (30 to 60 mmol/L), genetic testing may help to confirm or exclude the diagnosis. In patients with two CF-causing mutations on separate chromosomes, no further diagnostic testing is necessary, and CF diagnosis is confirmed. For patients without CF-causing mutations, no further testing is indicated as CF is unlikely. In patients with undefined CFTR mutations or CFTR mutations of varying clinical consequence, the clinician should pursue further testing if clinical features are present; this may entail NPD (nasal potential difference), ICM (intestinal current measurement), semen analysis, or pancreatic function testing.[1]

Interfering Factors

False positives are rare, but sweat chloride may elevate falsely in other pathologic syndromes and situations, including:

• Improper testing technique
• Atopic dermatitis
• Untreated adrenal insufficiency
• Glycogen storage disease
• Panhypopituitarism
• Hereditary nephrogenic diabetes insipidus
• Hypothyroidism
• Pancreatitis
• Malnutrition
• Mucopolysaccharidosis
• Ectodermal dysplasia
• Prostaglandin E1 infusion

Newborns may not produce enough sweat and may need to wait until later in infancy before an adequate sweat quantity can be collected. Infants with a positive newborn screen can undergo testing as early as 2 days of life. However, the Cystic Fibrosis Foundation recommends waiting until the child is 10 days old. For premature infants, testing should wait until they are 2 kilograms in size and greater than 36 weeks corrected gestational age, if possible.[4][5]

Patient Safety and Education

Sweat testing with QPIT is a quick, painless, safe, and reliable test used for CF diagnosis. The electrical stimulation is painless and does not cause discomfort. For safety reasons, the iontophoretic current source needs to be battery powered. Inspection for current control and leakage must be periodically performed by biomedical engineering according to the manufacturer’s recommendations. After a positive result, either a second testing sample is collected, or genetic testing is performed to confirm the diagnosis. Testing is only performed at CFF accredited cystic fibrosis centers. Other methods of measuring CTFR function, including measuring the total osmolality, conductivity, and sodium or potassium levels of sweat are not recommended for the diagnosis.[2]

Further information about sweat testing for patients and families is available via the Cystic Fibrosis Foundation.

Clinical Significance

If there is clinical suspicion of a child having cystic fibrosis from newborn genetic screening, or if clinically suspected by a healthcare provider, a sweat test should be performed to confirm the diagnosis. The sweat test is considered to be the gold standard for diagnosing CF and used in conjunction with clinical findings and family history to make the diagnosis. Cystic fibrosis is a  lifelong condition with many healthcare ramifications. Given the evolving therapies for patients with cystic fibrosis, early diagnosis is vital to ensure prompt therapeutic intervention and clinical monitoring. Patients with cystic fibrosis are living longer lives than ever before, with the median age of survival being greater than 40 years in developed countries. Children with diagnosis later in life likely to have better outcome given the likelihood of residual CFTR function and a less severe phenotype.[6]