Clostridium Botulinum

Article Author:
Aman Tiwari
Article Editor:
Shivaraj Nagalli
Updated:
12/30/2019 3:15:29 PM
PubMed Link:
Clostridium Botulinum

Introduction

The genus Clostridium is among the largest bacterial genera comprising of about 180 species.[1] The more common clinically relevant Clostridium species are Clostridium botulinum, which causes botulism; Clostridium perfringens, which causes food poisoning, gas gangrene, and necrotizing fasciitis; Clostridium tetani which cause tetanus and Clostridium sordellii which causes fatal infections after medical abortions. Here we will be reviewing the Clostridium botulinum organism, which is an anaerobic gram-positive spore-forming rod and is the most common cause implicated in reversible flaccid paralysis. Other clostridial bacteria can also produce the toxin leading to botulism. Despite its potential fatality and rarity, the illness can masquerade as other illnesses making the diagnosis difficult. Several studies show that the secretion of the submandibular gland (SMG) inhibited by botulinum toxin A (BTXA). Toxicity is detectable on the rat SMG-C6 cell line.[2]

Etiology

Clostridium botulinum produces botulinum toxin and causes botulism, a rare disease manifested as various clinical syndromes ranging from food poisoning, wound infection to infant botulism. The term is derived from Latin word botulus, meaning sausage, as poorly cooked sausages were formerly associated with food poisoning.

  • C. botulinum is anaerobic gram-positive bacillus with subterminal spore.
  • It is ubiquitous, widely distributed as a saprophyte in soil, animal manure, vegetables, and sea mud.[3]

Epidemiology

The Centre for Disease Control and Prevention (CDC) has been monitoring cases of botulism in the United States since 1973. From years 2011 through 2015, an average of 162 annual cases were reported. These primarily included infant botulism at 71% to 88%, followed by foodborne botulism, wound botulism, and botulism of unknown origin. Except for rare, large outbreaks, the total number of botulism cases has remained relatively stable over the past ten years.[4][5]

Mortality from botulism is low. The mortality rate had decreased significantly from over 60% in the 1950s to a mere 3% in 2009. Most deaths reported are due to botulism of unknown origin, followed by foodborne, wound, and infant botulism (less than 1%).[6]

Pathophysiology

C. botulinum is non-invasive. Its pathogenesis is due to the production of potent neurotoxin 'botulinum toxin' (BT), probably the most toxic substance known to be lethal to humankind. The botulinum neurotoxin is the most potent toxin known till now, with as little as 30-100 ng potentially fatal.[7]

BT is a zinc-dependent protein of 150 kDa (100 kDa heavy chain and a 50 kDa light chain). Eight types of C. botulinum strains have been identified (A, B, C1, C2, D, E, F, and G ) based on the immunological differences in the toxins produced by them. All serotypes produce neurotoxin, except C2, which produces an enterotoxin.[8] BT types C and D are bacteriophage coded. BT differs from other exotoxins, as it gets produced intracellularly, not secreted, and appears outside only after the autolysis of the bacterial cell. The toxin is synthesized initially as a nontoxic protoxin. It requires trypsin or other proteolytic enzymes to convert it into an active form.

Therapeutic Uses

As BT produces flaccid paralysis, it can be used therapeutically for the treatment of spasmodic conditions such as strabismus, blepharospasm, and myoclonus.[8] Botulinum toxin also gets produced by other clostridia such as C. butyricum, C. baratti, and C. argentinense

Recovery: Blocking of acetylcholine release is permanent, but the action is short-lasting as the recovery occurs in 2 to 4 months once the new terminal axons sprout. Spores do not produce toxins. Toxin production, therefore, requires spore germination, which occurs in an anaerobic atmosphere. Spores do not normally germinate in the adult intestine; however, they may germinate in the intestine of infants.

Toxicokinetics

After entry, botulism toxin gets transported to the cholinergic nerve endings via the blood. It does not affect the central nervous system. BT binds to the acetylcholine receptors on the neuromuscular junctions, resulting in blockage of the release of the neurotransmitter leading to flaccid paralysis.

History and Physical

The manifestations of botulism are due to a decrease in the availability of acetylcholine in the cranial nerve and parasympathetic nerve terminals. Common symptoms include:

  • Diplopia, dysphasia, dysarthria
  • Descending symmetric flaccid paralysis of voluntary muscles
  • Loss of deep tendon reflexes
  • Constipation
  • There are no sensory or cognitive deficits
  • Respiratory muscle paralysis may lead to respiratory failure and death.[9]

Types of Botulism

  • Foodborne botulism

It results from the consumption of foods contaminated with preformed botulinum toxin. The most common source is homemade canned food. Most cases are sporadic; outbreaks are rare.

  • Wound botulism

It results from contamination of wounds with C. botulinum spores. It presents like foodborne botulism except for the absence of gastrointestinal features.

  • Infant botulism

It results from the ingestion of contaminated food (usually honey) with spores of C. botulinum in children of 1 year of age. Spores germinate, releasing the toxin. Manifestations include the inability to suck and swallow, weakened voice, ptosis, floppy neck, and extreme weakness and hence referred to as 'floppy baby syndrome.' It is a self-limiting condition, managed by supportive care and assisted feeding. However, rarely it can progress to generalized flaccidity, respiratory failure, and sudden death.

  • Adult intestinal botulism 

Rarely, in patients with suppressed normal flora, the colonized clostridial spores may germinate producing toxin.

  • Iatrogenic botulism 

It results from the injection of an overdose of toxin while used for therapeutic purposes.

Evaluation

The diagnosis of botulism includes isolation of the bacilli and demonstration of the toxin.

Isolation of the Bacilli

  • Gram staining of smears made from suspected food or feces-reveals gram-positive, non-capsulated bacilli with subterminal, oval, bulging spores.
  • It is motile by peritricate flagella.
  • Isolation is possible when cultures are on blood agar or Robertson's cooked meat (RCM) broth. Bacterial colonies appear as large, irregular, semi-transparent, hemolytic with a fimbriated border on blood agar. In RCM broth, turbidity occurs with meat particles turning black and production of foul odor with C. botulinum A, B, F (proteolytic), and turn pink with C. botulinum C, D, E (saccharolytic). Growth on culture media may be confirmed by Gram staining and biochemical tests.
  • The mere presence of bacilli in food or feces is of less significance. Toxin demonstration is more meaningful.
  • Serotyping is through type-specific antisera.

Toxin Demonstration (Mouse Bioassay)

Toxins are detectable in the specimens (serum, stool, sterile water or saline enema, gastric aspirates, wound material) or samples of ingested foods.

• Specimens get injected into a mouse that develops paralysis in 48 hours, which can be inhibited by prior administration of specific antitoxin.

• The sensitivity of the mouse bioassay varies inversely with the time elapsed between the onset of symptoms and sample collection.[10]

Treatment / Management

  • In sick patients, meticulous intensive care support is needed, such as mechanical ventilation, if respiratory paralysis develops.
  • Botulinum antitoxin: It should be administered immediately on clinical suspicion without waiting for laboratory confirmation. Earlier the administration better is the cure rate because antitoxin can neutralize the unbound free toxin molecules. However, once toxin binds to nerve endings, antitoxin has no role.
  • In wound botulism: suspected wounds and abscesses should be cleaned, debrided, and drained promptly. Antibiotics though C. botulinum is susceptible to penicillin; the role of antibiotics is not established.[11][12]

Differential Diagnosis

The conditions which can mimic Clostridium botulism are as follows: 

  • Guillain-Barre syndrome (GBS)
  • Myasthenia gravis
  • Amyotrophic lateral sclerosis
  • Stroke
  • Organ phosphorus poisoning
  • Lambert Eaton syndrome
  • Tick paralysis
  • Antimicrobial associated paralysis
  • Acute intermittent porphyria (AIP)
  • Shellfish poisoning

Prognosis

Diseases caused by clostridial bacteria have a good prognosis if the patient is diagnosed and treated in time.

Complications

  • Nosocomial infections
  • Urinary tract infection
  • Thrombophlebitis
  • Deep vein thrombosis
  • Pressure sores
  • Contractures

Deterrence and Patient Education

Proper canning is a strong recommendation; Advise on heating all canned food before consumption can prevent the adult form of botulism. Refraining from using honey for kids under two years of age can be considered to avoid infant botulism. 

Pearls and Other Issues

As most cases of botulism follow the consumption of inadequately canned or preserved food, control is achievable by proper canning and preservation. When an outbreak occurs, a prophylactic dose of antitoxin should be given intramuscularly to all who consumed the food article.

Active immunization has demonstrated to be effective. If immunization is needed, as in laboratory workers exposed to the risks, two injections of aluminum sulfate adsorbed toxoids may be given at an interval of ten weeks, followed by a booster a year later. Antitoxin may be tried for treatment. Polyvalent antiserum to types A, B, and E may be administered as soon as arriving at a clinical diagnosis. Supportive therapy with the maintenance of respiration is of equal or great importance.

Enhancing Healthcare Team Outcomes

An investigational pentavalent botulinum toxoid is available for the people at elevated risk for BoNT exposure, such as laboratory workers and military personnel. No FDA-approved vaccine exists for the general public. The pentavalent toxoid is not under consideration for public use due to cost, the number of required vaccinations, and the recent decline in immunogenicity.


References

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[2] Xie S,Xu H,Shan XF,Cai ZG, Botulinum toxin type A interrupts autophagic flux of submandibular gland. Bioscience reports. 2019 Jul 31;     [PubMed PMID: 31273059]
[3] Dickson EC,Shevky E, BOTULISM. STUDIES ON THE MANNER IN WHICH THE TOXIN OF CLOSTRIDIUM BOTULINUM ACTS UPON THE BODY : II. THE EFFECT UPON THE VOLUNTARY NERVOUS SYSTEM. The Journal of experimental medicine. 1923 Sep 30;     [PubMed PMID: 19868794]
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[9] Holmberg M,Krogseth SB,Grude N,Wian KA, A man with laboured breathing, abdominal pain and vomiting. Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke. 2018 Mar 20;     [PubMed PMID: 29557119]
[10] Le Maréchal C,Fourour S,Ballan V,Rouxel S,Souillard R,Chemaly M, Detection of Clostridium botulinum group III in environmental samples from farms by real-time PCR using four commercial DNA extraction kits. BMC research notes. 2018 Jul 4;     [PubMed PMID: 29973253]
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[12] Sobel J,Rao AK, Making the Best of the Evidence: Toward National Clinical Guidelines for Botulism. Clinical infectious diseases : an official publication of the Infectious Diseases Society of America. 2017 Dec 27;     [PubMed PMID: 29293933]