Bone is the primary anatomical structure comprising of the human skeletal system. Functionally, it assumes a significant mechanical role by the skeleton, and represents a stock of mineral salts to mobilize for maintenance of calcium and phosphorus homeostasis. It protects several vital organs (skull, vertebrae and rib cage). Through the medullary spaces, it hosts, the bone provides structural and functional support for hematopoiesis
Several classifications can be proposed to separate bone subtypes. Overall, there are five generalized varieties of human bones (long, short, flat, sesamoid, and irregular) and two architectural subtypes, cortical and trabecular bones. The focus of this topic is on the different microscopic structures and components of bone.
As a specialized connective tissue, bone is comprised of mainly type 1 collagen fibers and inorganic salts. Type 1 collagen is a non-calcified matrix designed to resist the tension experienced by the weight-bearing functions of bone. It forms from osteoblasts, the main cellular component of bone.
Osteoblasts function in the production, transport, and arrangement of the osteoid matrix. Furthermore, they initiate and regulate matrix mineralization and control the neighboring bone cells activities. Osteoblasts morphology is closely related to their metabolic status. They are spindle-shaped when quiescent. When active, they are polyhedral, with abundant basophilic cytoplasm intimately in contact with the bone. They have a conspicuous nucleolus and a prominent perinuclear halo (developed Golgi apparatus).
Once the osteoid is mineralized, the precursor cells get surrounded by organic intracellular substances called lacunae to become fully developed and matured into osteocytes. The mineral content is chiefly hydroxyapatite crystals composed of mainly calcium and phosphate ions but can occasionally have sodium and carbonite as well.
Osteocytes have a widespread distribution. Their cell processes are fundamental to allows osseous tissue to be responsive to the mechanical and metabolic organism constraints. Histologically, they have long and delicate cytoplasmic processes and small nuclei, not always visible in every plane of section. Their number, size, shape, and position vary according to bone type. In fact, in woven bone, they are numerous, large, and disorganized, whereas, in lamellar bone, they are fewer in number, smaller, spindle-shaped and architecturally regular.
The osteon, anatomically, is represented as the single functional unit of bone tissue. It is arranged with concentric lamellae of collagen fiber orientations around a central canal consisting of osteocyte’s arterial, venous, and nerve supply, is known as the Haversian canal. This system also consists of the canaliculi and Volkmann canal which allow for communication between neighboring osteocytes and communication between neighboring osteons, respectively.
With a mechanism responsible for building up bone, osteoclasts are the large, multinucleated cells on the surface of bones in pits called Howship lacunae responsible for removing calcium from bone by secreting proteolytic enzymes and hydrogen ions to dissolve the calcium hydroxyapatite crystals.
On average, osteoclasts have 12 nuclei, though the number may range from 2 to as many as 100. In their cytoplasm, interconnecting actin filaments produce a clear area between the cell membrane and the nuclei.
Architecturally, bone categorizes into two subtypes: cortical and trabecular bones. Similarly, the primary mechanical determinants of the strength of bone are specific for the subtype as described: width and porosity for cortical bone, and shape, width, connectivity, and anisotropy for trabecular bone. Further differentiations regarding the two subtypes are as follows:
There are three main functions of the human skeletal system classified into the mechanical, formation of hematopoietic cells, and metabolism.
The best way to thoroughly observe the microarchitecture of bone is to perform undecalcified bone histology, which can reveal the mineralized and cellular components of the bone as well as patterns regarding the physiological processes of bone formation and resorption. This process, in turn, allows for histological techniques such as fluorochrome analysis and histomorphometry to further probe and observe the specimens.
Performing undecalcified bone histology varies from the techniques utilized in conventional paraffin embedded methods; this is because of density and lower permeability of the tissue, demanding more time-consuming fixation and processing times. The tissue is first placed in 10% phosphate-buffered formalin solution. Using a band saw, the specimen is cut to size and then placed in an opaque container, submerged in ascending concentrations of ethanol from 70 to 100% for one week at a time, increasing in ten percent increments. Afterward, the specimen is cleared in butanol for one week. It is imperative that the densities of the tissue and resin be similar., which can be done by using a test specimen beforehand and then modifying it the resin density afterward. Generally, a 2-hydroxyethyl methacrylate based preparation solution is the choice when assessing through light microscopy while methyl methacrylate is for immunohistochemistry.
Ground sectioning is prone to yield larger sections, ranging from 20 to 50 microns in thickness which makes it advantageous if intending to use fluorochrome analysis. The sledge microtome, on the other hand, tends to produce thin sections, more ideal for light microscopy.
Beyond the more widely used hematoxylin and eosin histochemical staining, several other stains are more advantageous in certain situations that warrant bone biopsies. This includes:
Through recent uses of transmission electron microscopy, ion-milled samples of cortical bone have demonstrated polycrystalline mineral structures enshrouding the collagen fibrils. Approximately 80% of the minerals, composed of hydroxyapatite juxtaposed with type 1 collagen, are outside the fibrils. The remaining amount of minerals that resides inside the fibrils are shown to be in the gap zones. These gap zones are between the C and N terminals of the collinear collagen proteins in the fibrils. Their arrangement is as crystals that are not as large as those outside the fibrils.
Electron tomography is also an alternative that utilizes bright-field images to build 3D models. In tissue samples where the mass thickness is dominant, such as in bone, the lattice planes from the crystals produce Bragg diffractions that lead to visualizations of wavering thicknesses. High-angle annular dark-field (HAADF) imaging, or Z-contrast imaging, is, likewise, another way of conceptualizing tomographic images of osseous tissue and is very useful in understanding the 3D relationship amongst the different biochemical systems at play.
Bone tissue is susceptible to a myriad of pathologies that may range from etiologies of embryological, metabolic, autoimmune, neoplastic, or idiopathic origins. These include, but are not limited to, the conditions discussed below.
Achondroplasia is a genetic disorder commonly associated as a cause of dwarfism. Individuals affected may present with short extremities due to decreased development of endochondral bone; this is the type of bone that is responsible for the growth of long bones in the body. This formation begins with the construction of cartilage matrices which are eventually supplanted by new forming bone. However, clinically, these patients present with normal-sized head and chests. This presentation is because the development of intramembranous bone is unaffected. These bone processes are responsible for forming flat bones – skull, sternum, and rib cage. They do not require a preexisting cartilage matrix to lay down new bone tissue in its place. Research has identified the cause as an activating mutation in fibroblast growth factor receptor 3, or FGFR3, gene. This gene is, under normal circumstances, inhibited to allow for standard bone growth. However, with a lack of inhibition of this gene, bone growth is arrested leading to the phenotype.
Paget disease of the bone is characterized to be an imbalance amongst the activities of osteoblasts and osteoclasts. Of unknown etiology, the condition only affects localized portions of the skeletal tissue, generally involving one or more neighboring bones rather than the diffuse skeletal system. The disease process has three different stages. Osteoclast activity first increases, leading to a higher breakdown of bone tissue than normal. From this, the negative feedback mechanism is activated leading to osteoblasts responding to the breakdown and beginning to lay down bone. Eventually, osteoblastic activity is uncontrolled and continues to lay down bone tissue far past the metabolic requirement yielding a thick, sclerotic bone that is susceptible to fractures. Clinical symptoms are a result of the end process of this overgrowth of bone such as bone pain from microfractures, hearing loss due to impingement on the vestibulocochlear nerve from excess bone, and lion-like facies following the involvement of the craniofacial bones. Alkaline phosphatase becomes elevated more than twice the normal level juxtaposed with the clinical features, in a patient should lead a physician to consider Paget as a diagnosis because an isolated elevation of alkaline phosphatase in adults above 40 years most commonly points to the disease. Bone specimens of these patients illustrate a mosaic pattern of lamellar bone representing the low threshold required to fracture.
If untreated, Paget disease of the bone can act as a risk factor for osteosarcoma, a malignant proliferation of osteoblasts. Much more common in teenagers than in the elderly, this skeletal neoplasm begins in the metaphysis of long bones with patients complaining of bone pain with swelling or as a pathologic fracture (a break in the bone caused by weakness of the bone through disease rather than trauma). According to the literature, the most common bones affected are the distal femur and the proximal tibia composing of bones of the knee joint. Radiological investigations of osteosarcomas depict a “sunburst” appearance of a destructive mass in affected areas. Due to the over-proliferation of osteoblasts, the periosteum is shown to be lifted, forming a Codman triangle. Levels of serum alkaline phosphatase and lactate dehydrogenase are also elevated in these patients as they are indicative of increased osteoblast activity. The degree of the elevation in these markers also determines the prognosis for patients, as higher levels signal more advanced disease. A biopsy is necessary for these patients. This biopsy can be either a closed biopsy through fine needle aspiration cytology (FNAC), a core needle biopsy, or an open/incisional biopsy which would allow an appropriate amount of tissue extraction for subsequent ancillary studies. Histological imaging demonstrates pleomorphic cells with the ability to produce osteoid, confirming the diagnosis.
|||Kelly PJ, Anatomy, physiology, and pathology of the blood supply of bones. The Journal of bone and joint surgery. American volume. 1968 Jun; [PubMed PMID: 4872730]|
|||Neve A,Corrado A,Cantatore FP, Osteoblast physiology in normal and pathological conditions. Cell and tissue research. 2011 Feb [PubMed PMID: 21120535]|
|||Camozzi V,Vescini F,Luisetto G,Moro L, Bone organic matrix components: their roles in skeletal physiology. Journal of endocrinological investigation. 2010 [PubMed PMID: 20938220]|
|||Allori AC,Sailon AM,Warren SM, Biological basis of bone formation, remodeling, and repair-part II: extracellular matrix. Tissue engineering. Part B, Reviews. 2008 Sep [PubMed PMID: 19183102]|
|||Cowan PT,Kahai P, Anatomy, Bones 2019 Jan; [PubMed PMID: 30725884]|
|||Unal M,Cingoz F,Bagcioglu C,Sozer Y,Akkus O, Interrelationships between electrical, mechanical and hydration properties of cortical bone. Journal of the mechanical behavior of biomedical materials. 2018 Jan; [PubMed PMID: 28888142]|
|||Bonewald LF, The amazing osteocyte. Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 2011 Feb [PubMed PMID: 21254230]|
|||Rochefort GY,Pallu S,Benhamou CL, Osteocyte: the unrecognized side of bone tissue. Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA. 2010 Sep [PubMed PMID: 20204595]|
|||Marks SC Jr,Popoff SN, Bone cell biology: the regulation of development, structure, and function in the skeleton. The American journal of anatomy. 1988 Sep [PubMed PMID: 3055928]|
|||Oftadeh R,Perez-Viloria M,Villa-Camacho JC,Vaziri A,Nazarian A, Biomechanics and mechanobiology of trabecular bone: a review. Journal of biomechanical engineering. 2015 Jan; [PubMed PMID: 25412137]|
|||Florencio-Silva R,Sasso GR,Sasso-Cerri E,Simões MJ,Cerri PS, Biology of Bone Tissue: Structure, Function, and Factors That Influence Bone Cells. BioMed research international. 2015; [PubMed PMID: 26247020]|
|||Goldschlager T,Abdelkader A,Kerr J,Boundy I,Jenkin G, Undecalcified bone preparation for histology, histomorphometry and fluorochrome analysis. Journal of visualized experiments : JoVE. 2010 Jan 8; [PubMed PMID: 20062000]|
|||Villanueva AR,Kujawa M,Mathews CH,Parfitt AM, Identification of the mineralization front: comparison of a modified toluidine blue stain with tetracycline fluorescence. Metabolic bone disease [PubMed PMID: 6200748]|
|||Bergmann B,Mölne J,Gjertsson I, The Bone-Inflammation-Cartilage (BIC) Stain: A Novel Staining Method Combining Safranin O and Van Gieson's Stains. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. 2015 Sep; [PubMed PMID: 26021335]|
|||Zhang Q,Cao L,Miao XD,Wu YP, Diagnostic value of TRACP5b expression in patients with bone tumors. Journal of biological regulators and homeostatic agents. 2019 Mar-Apr; [PubMed PMID: 30973000]|
|||Hou XF,Fan DW,Sun CG,Chen ZQ, Recombinant human bone morphogenetic protein-2-induced ossification of the ligamentum flavum in rats and the associated global modification of histone H3. Journal of neurosurgery. Spine. 2014 Sep; [PubMed PMID: 24949905]|
|||Chu ZM,Li HB,Sun SX,Jiang YC,Wang B,Dong YF, Melatonin promotes osteoblast differentiation of bone marrow mesenchymal stem cells in aged rats. European review for medical and pharmacological sciences. 2017 Oct; [PubMed PMID: 29077147]|
|||Georgiadis M,Müller R,Schneider P, Techniques to assess bone ultrastructure organization: orientation and arrangement of mineralized collagen fibrils. Journal of the Royal Society, Interface. 2016 Jun; [PubMed PMID: 27335222]|
|||McNally E,Nan F,Botton GA,Schwarcz HP, Scanning transmission electron microscopic tomography of cortical bone using Z-contrast imaging. Micron (Oxford, England : 1993). 2013 Jun; [PubMed PMID: 23545162]|
|||Daugherty A, Achondroplasia: Etiology, Clinical Presentation, and Management. Neonatal network : NN. 2017 Nov 1; [PubMed PMID: 29185944]|
|||Paul Tuck S,Layfield R,Walker J,Mekkayil B,Francis R, Adult Paget's disease of bone: a review. Rheumatology (Oxford, England). 2017 Dec 1; [PubMed PMID: 28339664]|
|||Kundu ZS, Classification, imaging, biopsy and staging of osteosarcoma. Indian journal of orthopaedics. 2014 May; [PubMed PMID: 24932027]|