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Pancytopenia


Pancytopenia

Article Author:
Shalini Chiravuri
Article Editor:
Orlando De Jesus
Updated:
11/20/2020 9:26:33 PM
For CME on this topic:
Pancytopenia CME
PubMed Link:
Pancytopenia

Introduction

Pancytopenia is a hematologic condition characterized by a decrease in all three peripheral blood cell lines. It is characterized by the hemoglobin of less than 11.5 g/dL in women and 13.5 g/dL in men, platelets of less than 150,000 per mcL, and leukocytes of less than 4000 per ml (or absolute neutrophil count of less than 1500-1800 per ml).[1][2]

Leukopenia is primarily seen as neutropenia since neutrophils constitute the majority of the leukocytes. Pancytopenia is not a disease, but a manifestation of other underlying conditions.[3] It is associated with multiple benign and malignant conditions.[1] Pancytopenia could be a result of decreased production of the cells or increased destruction. Anyone presenting with pancytopenia should undergo a thorough evaluation to identify the underlying etiology.

Etiology

The etiology of pancytopenia can be broadly categorized as a central type that involves disorders of production or a peripheral type that involves disorders of increased destruction.[4] These causes could contribute to the pancytopenia independently or as a combination.

Decreased production (central type): Pancytopenia due to decreased production is mostly secondary to nutritional deficiencies. Pancytopenia caused by bone marrow failure is known as aplastic anemia. Aplastic anemia could be idiopathic/autoimmune, secondary to infections (such as parvovirus B19, hepatitis, human immunodeficiency virus (HIV), cytomegalovirus, or Epstein-Barr virus), after drug toxicity, or chemotherapeutic agents (methotrexate, dapsone, carbimazole, carbamazepine, chloramphenicol). Pancytopenia can also be related to inadequate intake (as seen in eating disorders and alcoholics) or malabsorption.[5] The production of cell lines is also impaired when the bone marrow is infiltrated by malignancies (lymphoma, leukemia, multiple myeloma) or granulomatous disorders. Metastatic tumors can also cause bone marrow replacement late in the disease, thus producing pancytopenia.

Increased destruction (peripheral type): Peripheral destruction of cells can be associated with many autoimmune conditions (such as systemic lupus erythematosus, rheumatoid arthritis) and splenic sequestration (alcoholic liver cirrhosis, HIV, tuberculosis, malaria). Hypersplenism affects more frequently the platelets and erythrocytes than leukocytes.

With the current Covid-19 pandemic, pancytopenia has been reported secondary to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2).[6][7][8][9][10][11][12] Bone marrow aspiration had shown viral infection and infiltration with SARS-CoV-2.[13]

There is a subset of patients who have unexplained cytopenia despite extensive workup, classified as idiopathic cytopenias of unknown significance.[14]

Epidemiology

Pancytopenia incidence has a bi-modal presentation seen in children and adults frequently in the 3rd and 4th decades. Literature has reported a bw=etween 1.4 and 2.6 to 1 male to female predominance.[3][2] While conditions such as multiple myeloma and myelodysplastic syndrome are more prevalent in older patients, acute leukemia and parvovirus B19 infection are more common in younger patients.

In North America, the most common etiologies are myeloid neoplasms (acute myeloid leukemia, myelodysplasia, non-Hodgkin lymphoma, hairy cell leukemia, and precursor B acute lymphoblastic leukemia), followed by aplastic anemia, megaloblastic anemia, and HIV infections.[15]

Geographic and socio-cultural influences determine the major causes of pancytopenia, especially for megaloblastic anemia.[15][16] For megaloblastic anemia, there is usually no gender predominance.[2] There seem to be more cases in the East than the West, most likely due to the higher incidence of infections and drugs causing pancytopenia used in developing countries.

A study from 2013 in India showed that the most common causes are hypersplenism, infections, myelosuppression (cancer, chemotherapy, drug toxicity, or radiotherapy), and megaloblastic anemia.[3] A previous study in India showed megaloblastic anemia as the most frequent cause, followed by aplastic anemia.[16] In Mexico, the most common causes are myelodysplastic syndromes and megaloblastic anemia, followed by acute myeloblastic leukemia, acute lymphoblastic leukemia, hypersplenism, and aplastic anemia. In Turkey, the most common cause is megaloblastic anemia, followed by acute myeloid leukemia and aplastic anemia.[2]

Pathophysiology

The pathophysiology of aplastic anemia is an autoimmune-mediated T cell activation, which leads to the destruction of the hematopoietic stem cells. Bone marrow suppression is also caused by direct cytotoxic effects of medications such as methotrexate, anticonvulsants, and chemotherapeutic agents. Ineffective hematopoiesis is seen in the bone marrow of myelodysplastic syndrome.

Sepsis causes pancytopenias through several mechanisms (marrow suppression, hypersplenism, and consumptive coagulopathy), which usually act in combination. The virus causes pancytopenia through several mechanisms with modulation of the hematopoietic stem cells.[17] A massive cytokine storm syndrome had been implicated in cases of SARS-CoV-2.[7][9][12][18][19] Paroxysmal nocturnal hemoglobinuria is a genetic disease caused by the absence of glycophosphatidylinositol-linked proteins, such as CD55 and CD59, which prevent complement-mediated destruction of the cells. It involves the mutation of phosphatidylinositol glycan class A proteins.

History and Physical

The clinical presentation can be variable, with mild pancytopenia being asymptomatic to life-threatening emergencies in severe pancytopenia. Patients can present with manifestations of any of the decreased cell lines. Anemia can present as shortness of breath, fatigue, chest pain. Leukopenia manifests as increased infections, while thrombocytopenia presents with bruising, petechiae, and propensity for bleeding. Patients with severe neutropenia can present with severe infections. Patients with underlying liver disease can present with anorexia, nausea, or lethargy. Patients with splenic sequestration can present with left upper quadrant pain. Constitutional symptoms can be seen in patients with underlying autoimmune disorders or malignancies.

History is of utmost importance in the evaluation of pancytopenia. This should include investigating symptoms of autoimmune conditions, malignancies, recent infections, medications, chemotherapy, or radiation therapy. A detailed history of nutritional status should be taken. It should be noted that the presentation of malabsorption could be subtle, and pancytopenia may be the only presentation. Family history should be taken into account as well for inherited aplastic anemia. 

Physical examination may reveal pallor, petechiae, ulcers, rash. Signs of underlying liver disease may be seen in patients with cirrhosis. Splenomegaly may be seen in patients with splenic sequestration. Lymphadenopathy can be seen in patients with infections and lymphoma. Attention must be paid to signs of nutritional deficiencies in patients with eating disorders and alcoholism. The neurological examination is essential as it may show impairment of proprioception with a positive Romberg test and ataxia, suggesting subacute combined degeneration of the spinal cord secondary to vitamin B12 (cobalamin) deficiency and macrocytic anemia.[20]

Evaluation

Initial workup includes a complete blood count, along with reticulocyte count. This will help to determine if the pancytopenia is secondary to decreased production. The mean corpuscular volume would point towards megaloblastic anemia. A peripheral blood smear can show abnormal cells such as blasts, dysplastic leukocytes, and immature cells. The workup should also include vitamin B12 and folate levels, liver chemistry, lactate dehydrogenase. Infectious workup should be done as pancytopenia can be associated with infections such as HIV, malaria, and tuberculosis.[3][21][22][23]

In pancytopenia cases secondary to an acute viral infection, no further workup should be performed as most resolved rapidly. Follow-up laboratories can be performed to confirm the resolution of the pancytopenia. Similarly, in severe infections with sepsis, further workup should not be performed as the pancytopenia is most likely the sepsis result. The termination of the infection and sepsis will correct the pancytopenia.

Further evaluation for undiagnosed hepatitis and autoimmune conditions or malignancies should be pursued if suspected.[24] Serum calcium and parathyroid hormone levels can help patients with a negative workup, as there are cases of hyperparathyroidism causing pancytopenia.[25][26] A thyroid profile should also be obtained as hyperthyroidism is associated with pancytopenia.[27][28]

Bone marrow aspiration and biopsy can be done if no specific etiology is found to evaluate the status of the bone marrow stem cells. Bone marrow aspiration establishes the diagnosis for pancytopenia in 75% of cases.[1][15][29] The most common etiologies found are hypoplastic marrow, followed by megaloblastic anemia and hematological malignancies. Pathological examination of the bone marrow biopsy is helpful in malignant etiologies. It can show a clonal population of cells, primary/secondary malignant cells, acellular marrow, fibroblasts, granulomas from tuberculosis, sarcoidosis, or fungal infections.

Treatment / Management

Treatment is based on the underlying etiology for the pancytopenia. Nutritional deficiencies should be corrected. Any offending drug should be discontinued. Treatment for infections such as HIV or tuberculosis should be started immediately. If an autoimmune condition or malignancy is diagnosed, it should be treated. Aplastic anemia secondary to viral infections such as parvovirus is transient and symptomatic treatment should suffice. For patients with severe aplastic anemia, treatment options could include hematopoietic stem cell transplant and immunosuppression. Hematology referral should be sought for these patients.

Supportive care for patients includes red blood cell transfusion for anemia to alleviate symptoms and perfuse vital structures. Platelet transfusion is indicated for thrombocytopenia of less than 10,000 per mcL to prevent spontaneous intracranial bleeding. Prompt initiation of broad-spectrum antibiotic therapy is recommended for patients with neutropenic fever or severe neutropenia with an absolute neutrophil count of less than 500 per ml due to the risk of septic death.

Differential Diagnosis

Multiple conditions can present with pancytopenia; hence, when someone shows with pancytopenia, a complete evaluation is performed to detect the cause of pancytopenia. Bone marrow disorders such as aplastic anemia, myelodysplastic syndrome, acute leukemia, myelofibrosis, megaloblastic anemia, paroxysmal nocturnal hemoglobinuria, and Fanconi's anemia can present with pancytopenia. Fanconi anemia is the most common congenital cause of bone marrow failure with an autosomal recessive inheritance pattern. In myelofibrosis, there is a replacement of the bone marrow cells with fibrotic tissue. Malignancies such as lymphoma, multiple myeloma, and hairy cell leukemia can also present with pancytopenia. Non-bone marrow conditions present with pancytopenia include systemic lupus erythematosus, infections (such as parvovirus B19, Epstein Barr virus, HIV, hepatitis, leishmaniasis, tuberculosis, malaria, and histoplasmosis).[3]

Prognosis

The prognosis of pancytopenia depends on the underlying condition. The prognosis is excellent in conditions like viral infections, where the pancytopenia improves without any intervention. The prognosis in patients with myelodysplastic syndrome depends on the degree of pancytopenia and the percentage of blasts in the marrow. Patients receiving chemotherapy or drugs causing pancytopenia (methotrexate, linezolid, or anticonvulsants) may have to discontinue the treatment if alternative agents are available. Most of the time, pancytopenia is reversible with the discontinuation of the therapy.[30]

Complications

Complications of pancytopenia include increased risk of infections, life-threatening anemia, and bleeding. Patients presenting with fever will need broad-spectrum antibiotics and antifungals, with pan cultures. Supportive transfusions with packed red blood cells and platelets should be initiated promptly if severe anemia or thrombocytopenia with bleeding is present. Other complications include tumor lysis syndrome seen in patients receiving chemotherapy for substantial tumors like high-grade lymphoma and acute leukemia.[31]

Consultations

Patients with pancytopenia will need a hematology/oncologist consult. Additionally, patients with infections may need an infectious disease consult. Patients with rheumatological conditions and on treatment with methotrexate will need a rheumatology consult. If patients have undiagnosed or newly diagnosed thyroid or hypercalcemia, these patients will need an endocrinology consult.

Deterrence and Patient Education

Patients should be educated about the adverse reactions and toxicities of medications and over the counter supplements. The importance of periodic evaluations and blood work must be explained to a patient if started on drugs such as methotrexate or linezolid. Patients with underlying hematologic malignancies (such as multiple myeloma, myelodysplastic syndrome, lymphoma, or acute leukemias) should be cautioned about the possibility of pancytopenia.

If a patient is found with pancytopenia, the patient should be counseled on the complications of pancytopenia and increased risk for infections, bleeding, and manifestations of anemia. These patients should also be advised against using any other medications that may worsen pancytopenia.

Enhancing Healthcare Team Outcomes

Pancytopenia is a tricky manifestation with multiple etiological factors. Patients can present with symptoms of anemia, thrombocytopenia, and leukopenia. History taking is essential in this condition to determine the underlying cause of pancytopenia. While the hospitalist or the primary care provider is always the first physician to contact the patient, it is imperative to involve multiple teams and specialists such as hematologists/oncologists, rheumatologists, pathologists, radiologists, and pharmacists. Hematologists help in the diagnosis and management of pancytopenia. They can also perform bone marrow biopsy if the etiology is inconclusive. Pathologists and hematologists work together sometimes in these patients. Rheumatologists play a role in managing pancytopenia secondary to autoimmune diseases or side effects of medications used for autoimmune diseases. Pharmacists help with medications and dose-dependent adverse reactions. Nurses play a huge role in managing these patients; not only do they monitor the patient's vitals (especially fever), but they also help with patient counseling and education about the adverse effects of the potential culprit drugs. 

While pancytopenia treatment depends on the underlying cause, improved outcomes are seen with prompt consultation with an interprofessional group of specialists.


References

[1] Vargas-Carretero CJ,Fernandez-Vargas OE,Ron-Magaña AL,Padilla-Ortega JA,Ron-Guerrero CS,Barrera-Chairez E, Etiology and clinico-hematological profile of pancytopenia: experience of a Mexican Tertiary Care Center and review of the literature. Hematology (Amsterdam, Netherlands). 2019 Dec;     [PubMed PMID: 30890036]
[2] Das Makheja K,Kumar Maheshwari B,Arain S,Kumar S,Kumari S,Vikash, The common causes leading to pancytopenia in patients presenting to tertiary care hospital. Pakistan journal of medical sciences. 2013 Sep     [PubMed PMID: 24353701]
[3] Jain A,Naniwadekar M, An etiological reappraisal of pancytopenia - largest series reported to date from a single tertiary care teaching hospital. BMC hematology. 2013 Nov 6     [PubMed PMID: 24238033]
[4] Gnanaraj J,Parnes A,Francis CW,Go RS,Takemoto CM,Hashmi SK, Approach to pancytopenia: Diagnostic algorithm for clinical hematologists. Blood reviews. 2018 Sep;     [PubMed PMID: 29555368]
[5] Takeshima M,Ishikawa H,Kitadate A,Sasaki R,Kobayashi T,Nanjyo H,Kanbayashi T,Shimizu T, Anorexia nervosa-associated pancytopenia mimicking idiopathic aplastic anemia: a case report. BMC psychiatry. 2018 May 25;     [PubMed PMID: 29801443]
[6] Martín Pozuelo Ruiz de Pascual R,López Pardo P,López-Dóriga Bonnardeaux P, Pancytopenia during SARS-CoV-2 infection. Medicina clinica. 2020 Jul 4     [PubMed PMID: 32753109]
[7] Zhao Y,He J,Wang J,Li WM,Xu M,Yu X,Wu W,Sun C,Xu Z,Zhang W,Hu Y,Huang H, Development of pancytopenia in a patient with COVID-19. Journal of medical virology. 2020 Sep 29     [PubMed PMID: 32990983]
[8] Karki S,Rawal SB,Malla S,Rayamajhi J,Thapa BB, A case report on spontaneous hemoperitoneum in COVID-19 patient. International journal of surgery case reports. 2020     [PubMed PMID: 32959008]
[9] Mehta P,McAuley DF,Brown M,Sanchez E,Tattersall RS,Manson JJ, COVID-19: consider cytokine storm syndromes and immunosuppression. Lancet (London, England). 2020 Mar 28     [PubMed PMID: 32192578]
[10] Tang N,Li D,Wang X,Sun Z, Abnormal coagulation parameters are associated with poor prognosis in patients with novel coronavirus pneumonia. Journal of thrombosis and haemostasis : JTH. 2020 Apr     [PubMed PMID: 32073213]
[11] Al-Samkari H,Karp Leaf RS,Dzik WH,Carlson JCT,Fogerty AE,Waheed A,Goodarzi K,Bendapudi PK,Bornikova L,Gupta S,Leaf DE,Kuter DJ,Rosovsky RP, COVID-19 and coagulation: bleeding and thrombotic manifestations of SARS-CoV-2 infection. Blood. 2020 Jul 23     [PubMed PMID: 32492712]
[12]     [PubMed PMID: 32637095]
[13] Issa N,Lacassin F,Camou F, First case of persistent pancytopenia associated with SARS-CoV-2 bone marrow infiltration in an immunocompromised patient. Annals of oncology : official journal of the European Society for Medical Oncology. 2020 Oct     [PubMed PMID: 32615155]
[14] Steensma DP, The Clinical Challenge of Idiopathic Cytopenias of Undetermined Significance (ICUS) and Clonal Cytopenias of Undetermined Significance (CCUS). Current hematologic malignancy reports. 2019 Dec;     [PubMed PMID: 31696381]
[15] Devitt KA,Lunde JH,Lewis MR, New onset pancytopenia in adults: a review of underlying pathologies and their associated clinical and laboratory findings. Leukemia & lymphoma. 2014 May     [PubMed PMID: 23829306]
[16] Khunger JM,Arulselvi S,Sharma U,Ranga S,Talib VH, Pancytopenia--a clinico haematological study of 200 cases. Indian journal of pathology & microbiology. 2002 Jul     [PubMed PMID: 12785191]
[17] King KY,Goodell MA, Inflammatory modulation of HSCs: viewing the HSC as a foundation for the immune response. Nature reviews. Immunology. 2011 Sep 9     [PubMed PMID: 21904387]
[18] Huang C,Wang Y,Li X,Ren L,Zhao J,Hu Y,Zhang L,Fan G,Xu J,Gu X,Cheng Z,Yu T,Xia J,Wei Y,Wu W,Xie X,Yin W,Li H,Liu M,Xiao Y,Gao H,Guo L,Xie J,Wang G,Jiang R,Gao Z,Jin Q,Wang J,Cao B, Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet (London, England). 2020 Feb 15     [PubMed PMID: 31986264]
[19] Tveiten H,Aukrust P,Lehne G,Rodriguez JR,Skjønsberg OH, Haemophagocytic lymphohistiocytosis in COVID-19 cases? Tidsskrift for den Norske laegeforening : tidsskrift for praktisk medicin, ny raekke. 2020 Apr 21     [PubMed PMID: 32321221]
[20]     [PubMed PMID: 33023511]
[21] Santiago-Rodríguez EJ,Mayor AM,Fernández-Santos DM,Hunter-Mellado RF, Profile of HIV-Infected Hispanics with Pancytopenia. International journal of environmental research and public health. 2015 Dec 22;     [PubMed PMID: 26703689]
[22] Tiresse N,Allaoui M, [Multifocal tuberculosis revealed by pancytopenia: about a case]. The Pan African medical journal. 2018;     [PubMed PMID: 31011393]
[23] Achi HV,Ahui BJ,Anon JC,Kouassi BA,Dje-Bi H,Kininlman H, [Pancytopenia: a severe complication of miliary tuberculosis]. Revue des maladies respiratoires. 2013 Jan;     [PubMed PMID: 23318187]
[24] Sawada K,Takai A,Yamada T,Araki O,Yamauchi Y,Eso Y,Takahashi K,Shindo T,Sakurai T,Ueda Y,Seno H, Hepatitis-associated Aplastic Anemia with Rapid Progression of Liver Fibrosis Due to Repeated Hepatitis. Internal medicine (Tokyo, Japan). 2020 Apr 15;     [PubMed PMID: 31875639]
[25] Opie J,Omar F,Huang HC,Sandler L,Ross I, Primary hyperparathyroidism manifesting with pancytopenia. Pathology. 2017 Apr;     [PubMed PMID: 28267996]
[26] Lim DJ,Oh EJ,Park CW,Kwon HS,Hong EJ,Yoon KH,Kang MI,Cha BY,Lee KW,Son HY,Kang SK, Pancytopenia and secondary myelofibrosis could be induced by primary hyperparathyroidism. International journal of laboratory hematology. 2007 Dec;     [PubMed PMID: 17988303]
[27] Hamid OA,Fadul AM,Batia TB,Yassin MA, Graves' Disease-Related Pancytopenia Improved after Radioactive Iodine Ablation. Case reports in oncology. 2020 Jan-Apr;     [PubMed PMID: 32308594]
[28] Garla VV,Abdul Salim S,Yanes-Cardozo LL, Pancytopenia: a rare complication of Graves' disease. BMJ case reports. 2018 Mar 9;     [PubMed PMID: 29525760]
[29] Jha A,Sayami G,Adhikari RC,Panta AD,Jha R, Bone marrow examination in cases of pancytopenia. JNMA; journal of the Nepal Medical Association. 2008 Jan-Mar     [PubMed PMID: 18552886]
[30] Xiao N,Hao S,Zhang Y,Shao Z, Roles of immune responses in the pathogenesis of immunorelated pancytopenia. Scandinavian journal of immunology. 2020 Aug     [PubMed PMID: 32474938]
[31] Bagheri Z,Labbani-Motlagh Z,Mirjalili M,Karimzadeh I,Khalili H, Types and outcomes of cytopenia in critically ill patients. Journal of comparative effectiveness research. 2020 Jun     [PubMed PMID: 32495631]